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stain nuclei  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc stain nuclei
    Stain Nuclei, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclei+stain+dapi/DAPI/pm41496357-126-51-53
    Average 98 stars, based on 2155 article reviews
    stain nuclei - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Staining:

    Article Title: AAVR Expression is Essential for AAV Vector Transduction in Sensory Hair Cells
    Article Snippet: After the 20‐min AAV2 incubation, the culture medium was discarded, and the cells or explants were washed with pre‐warmed PBS before being fixed with 4% PFA at room temperature for 1 h. The amount of AAV2 particle attachment was then measured through immunolabeling of the intact AAV capsid using the A20 antibody (1:20, Progen, #61055) and counterstaining with phalloidin‐594 (1:200, Thermofisher, #A12381) to visualize HEI‐OC1 cell or sensory hair cell structures. .. DAPI was used to stain HEI‐OC1 cell nuclei (1:1000, Cell Signaling Technology, #4083). .. Seventy‐two hours post AAV2 incubation, the HEI‐OC1 cells were trypsinized (Gibco, #25200072), resuspended in PBS, and analyzed for green fluorescent protein (GFP) expression via flow cytometry.

    Article Title: Natural Fatty Acid Guards against Brain Endothelial Cell Death and Microvascular Pathology following Ischemic Insult in the Presence of Acute Hyperglycemia
    Article Snippet: The next day, washing (with PBS) followed incubation with secondary antibodies (conjugated with Alexa 488); rabbit anti-mouse 1:1000, Invitrogen, Waltham, MA, USA; and Goat anti-rabbit (1:1000), Proteintech, respectively. .. To stain the nuclei, we used DAPI #4083; 1:500; Cell Signaling Technology (Cambridge, UK). .. For 4-HNE staining, we incubated brain slices with anti-4-hydroxynonenal (4-HNE) 1:1000 (EMD Millipore, St. Louis, MO, USA) and anti-CD31 (557355) 1:1000 (Proteintech, USA).

    Article Title: AAVR Expression is Essential for AAV Vector Transduction in Sensory Hair Cells.
    Article Snippet: After the 20-min AAV2 incubation, the culture medium was discarded, and the cells or explants were washed with pre-warmed PBS before being fixed with 4% PFA at room temperature for 1 h. The amount of AAV2 particle attachment was then measured through immunolabeling of the intact AAV capsid using the A20 antibody (1:20, Progen, #61055) and counterstaining with phalloidin-594 (1:200, Thermofisher, #A12381) to visualize HEI-OC1 cell or sensory hair cell structures. .. DAPI was used to stain HEI-OC1 cell nuclei (1:1000, Cell Signaling Technology, #4083). .. Seventytwo hours post AAV2 incubation, the HEI-OC1 cells were trypsinized (Gibco, #25200072), resuspended in PBS, and analyzed for green fluorescent protein (GFP) expression via flow cytometry.

    Article Title: Cannflavin B ameliorates behavioural and neuronal systems alterations in adolescent rats exposed to prenatal valproic acid.
    Article Snippet: Sections were first washed in 1X Tris-Buffered Saline (TBS, 60.5 mM Tris, 87.6 mM NaCl pH 7.6), blocked for 2 h (10 % goat serum, 1 % BSA, 0.2 % Triton-X, 1X TBS), and incubated with mouse anti-Iba1 (ionized calcium-binding adaptor molecule 1 (Iba1), 1:200; Abcam, AB283319) primary antibody for 60 h at 4 oC. .. Following incubation, sections were washed in 1X TBS, blocked (5 % goat serum, 0.5 % BSA, 0.01 % Triton-X, 1X TBS), and incubated in an anti-mouse-Alexa 488 (1:200; Invitrogen) secondary antibody for 2 h. Slices were then washed, and on the last wash 0.4 μg/mL of DAPI (4’,6-diamidino-2-phenylindole) was added to stain nuclei (Cell Signaling, #4083). .. Brain slices were then mounted on slides using Prolong Gold (Thermo Fisher Scientific).

    Article Title: Cannflavin B ameliorates social and anxiety deficits and neuronal systems dysfunction in adolescent rats exposed to prenatal valproic acid
    Article Snippet: Following incubation, sections were washed in 1X TBS, blocked (5% goat serum, 0.5% BSA, 0.01% Triton-X, 1X TBS), and incubated in an anti-mouse-Alexa 488 (1:200; Invitrogen) secondary antibody for 2 hours. .. Slices were then washed, and on the last wash 0.4 μg/mL of DAPI was added to stain nuclei (Cell Signaling, #4083). .. Brain slices were then mounted on slides using Prolong Gold (Thermo Fisher Scientific).

    Article Title: Natural Fatty Acid Guards against Brain Endothelial Cell Death and Microvascular Pathology following Ischemic Insult in the Presence of Acute Hyperglycemia.
    Article Snippet: The next day, washing (with PBS) followed incubation with secondary antibodies (conjugated with Alexa 488); rabbit anti-mouse 1:1000, Invitrogen, Waltham, MA, USA; and Goat anti-rabbit (1:1000), Proteintech, respectively. .. To stain the nuclei, we used DAPI #4083; 1:500; Cell Signaling Technology (Cambridge, UK). .. For 4-HNE staining, we incubated brain slices with anti4-hydroxynonenal (4-HNE) 1:1000 (EMD Millipore, St. Louis, MO, USA) and anti-CD31 (557355) 1:1000 (Proteintech, USA).

    Incubation:

    Article Title: Cannflavin B ameliorates behavioural and neuronal systems alterations in adolescent rats exposed to prenatal valproic acid.
    Article Snippet: Sections were first washed in 1X Tris-Buffered Saline (TBS, 60.5 mM Tris, 87.6 mM NaCl pH 7.6), blocked for 2 h (10 % goat serum, 1 % BSA, 0.2 % Triton-X, 1X TBS), and incubated with mouse anti-Iba1 (ionized calcium-binding adaptor molecule 1 (Iba1), 1:200; Abcam, AB283319) primary antibody for 60 h at 4 oC. .. Following incubation, sections were washed in 1X TBS, blocked (5 % goat serum, 0.5 % BSA, 0.01 % Triton-X, 1X TBS), and incubated in an anti-mouse-Alexa 488 (1:200; Invitrogen) secondary antibody for 2 h. Slices were then washed, and on the last wash 0.4 μg/mL of DAPI (4’,6-diamidino-2-phenylindole) was added to stain nuclei (Cell Signaling, #4083). .. Brain slices were then mounted on slides using Prolong Gold (Thermo Fisher Scientific).



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